XB-IMG-131539
Xenbase Image ID: 131539
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Fig. 8. Additive effects of Xvent genes in gainand
loss-of-function. (A) Xvent genes ventralize
mesoderm in an additive fashion. Embryos were
either non-injected (Co) or microinjected
radially at the 4- to 8-cell stage with Xvent-1
(range 0.125-0.25 ng/blastomere) or Xvent-2
mRNA (range 0.37-075 ng/blastomere), or
injected with a mixture (same range of both).
The average dorsoanterior index (Kao and
Elinson, 1988) of embryos was 4.8 (uninjected
control, n=22), 4.4 (Xvent-1, n=34), 3.0 (Xvent-
2, n=47) and 2.6 (Xvent-1 + Xvent-2, n=33).
(B) Xvent genes function in an additive fashion
in downregulation of dorsal and upregulation of
ventral marker genes in DMZ. Embryos were
either non-injected (Co), or microinjected
radially at the 4- and 8-cell stage with the doses
indicated in ng/blastomere with Xvent-1 or
Xvent-2 mRNAs, or injected with a mixture.
Control (Co) VMZ and DMZs were explanted
at early gastrula stage and fixed immediately for
total RNA preparation. Expression of marker
genes indicated was assayed by RT-PCR.
(C) VPXvent-1/2 mRNA injections induce
dorsal marker genes additively. Embryos were
either non-injected (Co), or microinjected
radially at the 4- to 8-cell stage with the doses
indicated in ng/blastomere with VPXvent-1 or
VPXvent-2 mRNAs, or injected with a mixture.
Control (Co) DMZ and VMZs were explanted
at early gastrula stage and fixed immediately for
total RNA preparation. Expression of marker
genes indicated was assayed by RT-PCR.
Histone H4 was used for normalisation. Image published in: Onichtchouk D et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |