XB-IMG-131557
Xenbase Image ID: 131557
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Fig. 2. Spatio-temporal expression of Zic-r1
and Sox-2. Whole-mount in situ
hybridization analyses of Zic-r1 (labeled as
ZCR; A, C, E, G, I, K, M) and Sox-2
(labeled as SOX; B, D, F, H, J, L, N) at the
early gastrula stage (stage 10.5; A-D), the
early neurula stage (stage 14; E, F), the midneurula
(stage 16; G, H) and the larva stage
(stage 32; I, J). (C and D) show 10 mm
paraffin sections. The signals are detected in
superficial dorsal ectoderm but not in the
deep mesoderm. The arrow and arrowhead
in J show the branchial arch neural crest and
the olfactory placode, respectively.
(K-N) Radial injection of Chd mRNA (150
pg/blastomere; K, L) and of BMP-4 mRNA
(75 pg/blastomere; M, N) into the 4-cell
embryo. Chd injection induced Zic-r1 (P)
and Sox-2 (Q) expression in the animal caps
(excised at stage 9 and harvested at stage
11.5) but not the mesodermal marker Xbra
(O). Activin-treated animal caps (excised at
stage 9 and treated with 10 ng/ml activin until stage 11.5) for positive control for Xbra (O, inset). (P,Q, insets) Uninjected animal caps. The
arrowheads show the dorsal lip in A-D and the closing blastopore in E-H. Image published in: Mizuseki K et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |