Xenbase Image ID: 131559

Fig. 4. Zic-r1 injection promotes neural and neuronal differentiation in animal caps and in vivo. After 2 days culture, when siblings reached stages 38-39, animal cap explants injected with Zic-r1 mRNA (A) contained a large neural mass. V, ventricle-like cavities; arrow, roof plate-like thin layer. The control animal cap contained atypical epidermis (B). When siblings reached stage 17, Zic-r1-injected animal caps expressed the neural marker nrp-1 (C; detected by in situ hybridization). Inset (C) shows control animal caps. Injection of 200 pg Zic-r1 mRNA into one blastomere of the 2-cell embryo expanded expression of Xngnr-1 (D; primary neurons shown by triangles; arrow, midline), N-tubulin (E; note the width of lateral neuron bands, l) and slug (F; arrow, ectopic expression) and suppressed that of keratin (G, H) in vivo at neural plate stages. (I) Expression of hairy 2, which marks the midline and borders of neural plate (np) (Turner and Weintraub, 1994). The width of neural plate (arrows) is significantly increased on the injected side compared to the control side. epi, epidermis. The injected side (inj; cont, control side) was determined by coinjecting a GFP (D-F, I; not shown) or b-gal (arrows in G) mRNA. Image published in: Mizuseki K et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page