XB-IMG-131574
Xenbase Image ID: 131574
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Fig. 2. Molecular analysis of Xdazl mRNA expression. (A) Northern
blot of Xdazl RNA expression in adult tissues. Xdazl is shown in the
top panel, below is the same membrane stripped and reprobed with
EF1-a as a loading control. In the top panel, the size of the Xdazl
band is shown at the left. The tissues tested are labeled above each
lane. (B) RNase protection assay of Xdazl expression during
embryogenesis. RNA from four embryo equivalents of the indicated
stages was extracted and analyzed. Stages tested are indicated at the
top of each lane. Torula (yeast) total RNA was included as a negative
control. A probe for ODC2 was included as a loading control. The
ratio of Xdazl:ODC2 relative band intensities as determined by
Phosphorimager quantitation is shown below each lane. (C) RNase
protection assay of Xdazl mRNA expression in juvenile testis (J.
Testis) and ovary (J. Ovary). Positions of the Xdazl and ODC2
protected fragments are indicated by arrows. Included here are
examples of undigested probes (Probes) and two equivalents of
positive control stage VI oocytes. Arrows indicate the positions of
the protected fragments. Image published in: Houston DW et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |