Xenbase Image ID: 131577

Fig. 5. Expression of Xdazl mRNA during embryogenesis analyzed by in situ hybridization to sectioned material. (A) 8-cell embryo hybridized with Xdazl sense probes. The region of the germ plasm (gp) is shown by the arrow. The vegetal pole (vg) of the embryo is to the lower left. (B) Another section from the same embryo as (A) probed with antisense Xdazl probes. The yolk-free region of the germ plasm is stained. The vegetal pole (vg) is to the lower left. (C) Section from a stage 7 embryo hybridized with Xdazl antisense probes. Staining is seen in streaks near the vegetal pole. (D) Higher magnification of (C). (E) Control stage 7 section immunostained with anti-vimentin antibody Z10. The arrow indicates the vimentin-stained germ plasm. (F) Low power view of a section from a stage 10 embryo hybridized with Xdazl antisense probes. Staining reveals a number of pPGCs near the archenteron (a), indicated individually by arrowheads. (G) The upper panel shows a higher power view of one of the pPGCs in (F), showing perinuclear Xdazl staining (arrow). The lower panel is a section from a different embryo showing a similar staining pattern (arrow). (H) Control stage 10 section immunostained with Z10. The germ plasm is indicated (arrow) and surrounds a nucleus along the floor of the archenteron. Scale bars, 250 mm (C, F), 160 mm (D), 125 mm (E), 62.5 mm (A,B,G,H). n, nucleus. Image published in: Houston DW et al. (1998) Copyright © 1998. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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