Xenbase Image ID: 1316

Figure 1. FoxC1 is an indirect target of VegT and FoxC1 expression in the equatorial zone. A: VegT-depleted gastrulae (early gastrula, stage 10; mid gastrula, stage 10.5) showed a decrease of FoxC1 expression using real time RT-PCR compared to control sibling embryos. Levels of expression are normalized to ornithine decarboxylase (ODC).B: Using whole-mount in situ hybridization, FoxC1 was shown to be localized in the equatorial zone of wild-type embryos at the mid-gastrula stage (stage 11). The upper two half-embryos seen in hemisection through the blastopore; lower two whole embryos, viewing the blastopore. C: After CerS mRNA injection at the 2-cell stage, FoxC1 expression measured by real time RT-PCR is reduced in wild type gastrulae, at the early and mid-gastrula stage. D: The expression of FoxC1 in 1 whole embryo compared to batches of 10 wild type animal caps (Caps), 10 caps injected with 60 pg VegT mRNA at the two cell stage (Caps + VegT mRNA), 10 wild type caps treated with cycloheximide (Caps + cyclohex), and 10 caps injected with VegT mRNA and treated with cycloheximide (Caps + VegT + cyclohex). Wild-type animal caps do not express FoxC1, while injection with VegT mRNA induces FoxC1 expression. Injection of cycloheximide in addition to VegT mRNA into animal caps represses FoxC1 expression. Image published in: Cha JY et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue foxc1.S fkhl7, foxc1-a, foxc1-b, freac-3, freac3, igda, ihg1, irid1, rieg3, xfd-11 X. laevis Throughout NF stage 11 involuted ventral mesoderm marginal zone involuted dorsal mesoderm mesoderm Image source: Published Larger Image Printer Friendly View

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