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Fig. 1. Animal caps injected with vegetal cortical cytoplasm express Siamois and Xnr3 but not Xbra. Animal caps from embryos injected with vegetal cortical cytoplasm (VCC) or animal cortical cytoplasm (ACC) were analysed by RT-PCR at stage 10.5 for the expression of Siamois, Xnr3 and Xbra. Endogenous FGFR1 transcripts were used here as an internal standard. Analysis of the same samples by RNase protection gave similar results, as did independent northern analysis of Xnr3. + and – refers in this and the following figures to the presence or absence of reverse transcriptase, as a control for DNA contamination. WE, whole embryo; An, uninjected animal caps.

Image published in: Darras S et al. (1997)

Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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