XB-IMG-131632
Xenbase Image ID: 131632
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Fig. 4. Dorsalising activity of dorsal marginal zone cells, dorsal vegetal cells or vegetal
pole cells from UV-irradiated embryos. (A) Experimental strategy. Explants of ventral
marginal zone (VMZ) were taken at stage 10 from embryos injected with the lineage
tracer rhodamine lysinated dextran (RLDx). They were combined with either vegetal
pole from UV-treated embryos (UV Veg Pole), dorsal vegetal cells from stage 8.5-9
embryos (D. Veg) or stage 10 dorsal marginal zone (DMZ). The conjugates were
cultured until stage 30-35 and immunostained with the muscle antibody 12/101.
(B) Photographs of sections through conjugates of ventral marginal zone explants
(VMZ) with UV vegetal pole (top), dorsal vegetal cells (middle) or dorsal marginal zone
explants (bottom). While DMZ and dorsal vegetal cells have a dorsalising activity, UV
vegetal poles fail to dorsalise VMZ explants. Notochordal differentiation, monitored by
the presence of highly vacuolated cells, is found in the DMZ explants but never observed
in the dorsal vegetal explants. Left panels, 12/101 staining; right panels, position of
RLDx labelled cells. Image published in: Darras S et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |