XB-IMG-131633
Xenbase Image ID: 131633
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Fig. 5. Dorsalising activity of animal or vegetal pole cells injected
with Siamois or chordin mRNA. (A) Experimental strategy. Stage 10
ventral marginal cells (derived from embryos injected with RLDx)
were combined either with animal cap (stage 8.5-9) derived from
embryos previously injected in the animal pole with 900 pg of
chordin mRNA or 100 pg Siamois mRNA or with vegetal cap
derived from embryos injected similarly in the vegetal pole. The
conjugates were cultured until stage 30-35 and immunostained with
the muscle antibody 12/101. (B) Photographs of sections through
conjugates of VMZ explants with chordin mRNA injected animal
caps (chd an) or vegetal poles (chd veg), or with Siamois mRNAinjected
animal caps (Sia An) or vegetal poles (Sia veg). While
injection of chordin mRNA confers dorsalising activity to both
animal and vegetal pole cells, injection of Siamois mRNA leads to
the secretion of dorsalising signals in animal caps cells only. chd,
chordin; Sia, Siamois. Left panels: 12/101 staining; right panels:
RLDx labelled cells. Image published in: Darras S et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |