XB-IMG-131634
Xenbase Image ID: 131634
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Fig. 6. Activation of chordin and cerberus by Siamois is context dependent. (A) Cerberus
is a target of Siamois. 4-cell embryos were injected with 100 pg of Siamois mRNA in a
ventral vegetal location or with 100 pg of enR-Sia mRNA in a dorsal vegetal position.
Control and injected embryos were reared until stage 10 before excising ventral vegetal
cells when needed. Whole embryos (WE) and ventral vegetal explants from uninjected
(VVZ) or Siamois-injected embryos (VVZ+Sia) were analysed at stage 11 for cerberus
expression. (B) Chordin, but not cerberus, is activated by the VCC in animal caps. The
same cDNA samples as in Fig. 1 were subjected to RT-PCR analysis for the expression of
chordin and cerberus. Abbreviations are as in Fig. 1. (C) cerberus is expressed at the
vegetal pole of UV-irradiated embryos. Control or UV-irradiated (U.V.) whole embryos
(WE) as well as explants were analysed at stage 11 by RT-PCR. In the experiment shown
on the left panel, chordin expression was undetectable in the UV-irradiated whole
embryos (not shown). enR-Sia: embryos injected at the vegetal pole with 100 pg of enRSia
mRNA. Image published in: Darras S et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |