XB-IMG-131650
Xenbase Image ID: 131650
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Fig. 4. Injection of Dll3 RNA inhibits primary neuron formation in
Xenopus. (A-D) Neural plate stage Xenopus embryos (dorsal view,
posterior to the right) showing N-tubulin gene expression (purple)
identifying primary neurons, in medial (m), intermediate (i) and
lateral (l) locations. A single injection of 50 pg of lacZ RNA and 200
pg of X-Delta-1 or Dll3 RNA into a 2- or 4-cell embryo gave
localisation of the transcripts to different regions of the neural plate
as shown by X-gal staining (bracket). (A) Ectopic expression of XDelta-
1 RNA supresses primary neuron formation. The lateral stripe
of neurons (l) is absent in the region where X-Delta-1 RNA is
localised, indicated by Xgal-positive cells (bracket). (B-D) Ectopic
expression of Dll3 RNA supresses primary neuron formation. Lateral
and intermediate neurons are reduced in number (B) or absent (C)
where Dll3 RNA is localised (bracket). (D) Medial, intermediate and
anterior lateral neurons are absent where Dll3 RNA is localised
(bracket). Scale bar, 330 mm. Image published in: Dunwoodie SL et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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