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Fig. 5. Increasing amounts of RNA encoding Xbra-GR induce different types of mesoderm. Histological (A-C) and immunohistochemical (D-F) analyses of animal caps derived from embryos injected with increasing amounts of RNA encoding Xbra-GR. Animal caps were dissected at stage 8, treated with 10-6 M DEX, and cultured to stage 42 when they were fixed and sectioned at 7 mm for histological analysis or at 10 mm for immunohistochemical analysis. For histological analysis (A-C), the sections were stained by the Feulgen technique and with Light Green and Orange G. For immunohistochemical analysis (D-F), the sections were stained with 12/101 monoclonal antibody for muscle. (A,D) Untreated caps treated with DEX form atypical epidermis. (B,E) DEX-treated animal caps derived from embryos injected with 25 pg of RNA encoding Xbra-GR form ventral mesodermal vesicles, which contain mesothelium and mesenchyme. (C,F) DEX-treated animal caps derived from embryos injected with 400 pg of RNA encoding Xbra-GR form clumps of muscle cells. This muscle tissue is typical of caps derived from embryos injected with 100 pg of RNA encoding Xbra-GR or more. Image published in: Tada M et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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