Xenbase Image ID: 131690

Fig. 6. Effects of injected RNA encoding Xbra-GR in whole embryos. (A-D) One animal pole blastomere (tier A) of embryos at the 32-cell stage received injections of RNA encoding Xbra-GR (10 pg). Embryos were left untreated (A) or exposed to 10-6 M DEX at stage 10 (B-D). They were allowed to develop to stage 34. DEX-treated embryos typically possess tail-like protrusions (B,C). When protrusions are present in the head, embryos lack eyes (C) and when they occur near the tail, they contain fin-like structures (D). The tail-like protrusions are shown by arrows. Treatment with DEX at stage 10 was more effective than that at stage 8 or 12. (E-H) Embryos were injected in one blastomere of tier A (E,F) or tier C (G,H) at the 32-cell stage with 10 pg of RNA encoding Xbra-GR together with nucb-gal RNA. The embryos were treated with 10-6 M DEX at stage 10 (F,H) or were untreated (E,G), fixed at stage 34 and stained with X-gal. Embryos injected in tier A and treated with DEX possess tail-like protrusions (see Table 2). When Xbra-GR and nucb-gal RNAs were injected into a tier A blastomere in the absence of DEX, labelled cells were found in the surface ectoderm (E) or in neural tissues, but addition of DEX at stage 10 changed the fate of these cells causing them to form tail-like protrusions (98% of cases; n=67) (F). In contrast, following injection into a tier C blastomere, labelled cells were observed in the notochord or in the paraaxial cells (G) even after treatment with DEX (H). (I-L) Whole-mount immunostaining analysis of embryos injected with 10 pg of RNA encoding Xbra-GR into a tier A blastomere at the 32-cell stage. The embryos were left untreated (I,K) or treated with 10-6 M DEX at stage 10 (J,L), fixed at stage 34 and stained with MZ15 monoclonal antibody for notochord (I,J) or with 12/101 monoclonal antibody for muscle (K,L). Tail-like protrusions are negative for notochord (J), while they are positive for muscle (arrow in L). Image published in: Tada M et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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