Xenbase Image ID: 131691

Fig. 7. Stability of Xbra-GR protein in Xenopus embryos. (A) Immunoprecipitation analysis of in vitro translated protein derived from Xbra-HA, Xbra-GR-HA and GR-HA, all of which include repeated nonapeptides from the influenza hemagglutinin protein (HA), using anti-HA monoclonal antibody 12CA5 (see Materials and methods). Immunoprecipitates were analysed by 12% acrylamide gel electrophoresis followed by fluorography. Positions of molecular mass markers (´10-3) are indicated. (B) Western blotting analysis of Xbra-GR protein levels in Xenopus embryos. Animal caps from embryos injected with 400 pg of RNA encoding Xbra-GR-HA were dissected at stage 8, treated with 10-6 M DEX where appropriate and collected at the indicated stages. Two cap equivalents of protein were analysed by western blotting using the anti-HA antibody after 12% acrylamide gel electrophoresis. Positions of molecular mass markers (´10-3) are indicated. The band corresponding to the protein of Xbra-GR-HA is indicated by an arrowhead. Note that Xbra-GR-HA protein levels are stable in the absence of DEX but fall rapidly after its addition. Image published in: Tada M et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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