XB-IMG-131692
Xenbase Image ID: 131692
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Fig. 8. Competence of animal
caps to respond to mesoderm
inducers. (A) Embryos at the
one-cell stage were left
uninjected or injected with 100
pg of RNA encoding Xbra-GR.
Animal caps were dissected at
stage 8, 9, 10 or 11, then
treated with 100 ng/ml bFGF, 8
U/ml activin or 10-6 M DEX,
and cultured to stage 23, when
they were analysed for
expression of actin genes by
RNAase protection. Competence in response to bFGF and activin is gradually lost by the
early gastrula stage, whereas the response to Xbra persists. (B) Competence of animal caps
to respond to Xbra. Embryos at the one-cell stage were injected with 100 pg of RNA
encoding Xbra-GR. Animal caps were dissected at stage 8, treated with 10-6 M DEX at the
indicated stages, and cultured to stage 23, when they were analysed for expression of actin
genes by RNAase protection. Competence of animal caps to respond to Xbra persists
during until the late gastrula stage (stage 12). Image published in: Tada M et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |