XB-IMG-131693
Xenbase Image ID: 131693
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Fig. 9. Autoinduction of Xbra. (A) Autoinduction of Xbra in animal
pole explants. Embryos were injected with 25 pg of RNA encoding
Xbra-GR at the one-cell stage. Animal caps were dissected at stage
8, treated as appropriate with 10-6 M DEX and cultured to stage
10.5, when they were analysed for expression of Xbra by RNAase
protection. ODC serves as a loading control. (B,C) Autoinduction of
Xbra in whole embryos. Embryos were injected with 10 pg of RNA
encoding Xbra-GR into a tier A blastomere at the 32-cell stage. The
embryos were left untreated (B) or exposed to 10-6 M DEX at stage
10 (C) and allowed to develop to stage 11.5, when they were
analysed by whole-mount in situ hybridisation. Ectopic expression of
Xbra is seen in the prospective ectoderm. Image published in: Tada M et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |