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Xenbase Image ID: 131705

Fig. 2. Expression pattern of the Brat gene in Xenopus development. (A) A Developmental northern blot on total embryonic RNA revealed a single Brat transcript of approximately 3 kb that is expressed maternally and zygotically into mid-neurulation (Stage 15). Stages analyzed were egg, blastula (stages 7,9), gastrula (stage 11), neurula (stages 15,18) tailbud tadpole (stage 26) and swimming tadpole (stage 38). Five embryo equivalents were loaded per lane and RNA recovery and integrity was equal across the blot (not shown). (B) Localization of Brat mRNA in oocytes by whole-mount in situ hybridization. Brat mRNA is expressed throughout oogenesis and is localized to the vegetal pole by stage II. Pigmented oocytes (P) are shown in the upper panel, animal pole up, to orient the stain relative to the animal and vegetal pole. Albino oocytes (A) are shown in the lower panel to clearly display the domain occupied by Brat transcripts. During the in situ procedure, the cortex was partially removed from several stage VI oocytes to demonstrate that the bulk of Brat transcripts are sequestered in the cortex. Arrows indicate the edge where the cortex was torn off, revealing the underlying, unstained cytoplasm. (C) Vegetal localization of Brat mRNA in early cleavage stage embryos. A northern blot of RNA from animal and vegetal halves of stage 6 embryos. Seven explants or three embryos were analyzed and the blot was reprobed with Histone H4 to contrast the localization of Brat mRNA with that of a cytoplasmic mRNA.

Image published in: Horb ME and Thomsen GH (1997)

Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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