Xenbase Image ID: 131705
Fig. 2. Expression pattern of the Brat gene in Xenopus development.
(A) A Developmental northern blot on total embryonic RNA
revealed a single Brat transcript of approximately 3 kb that is
expressed maternally and zygotically into mid-neurulation (Stage
15). Stages analyzed were egg, blastula (stages 7,9), gastrula (stage
11), neurula (stages 15,18) tailbud tadpole (stage 26) and swimming
tadpole (stage 38). Five embryo equivalents were loaded per lane and
RNA recovery and integrity was equal across the blot (not shown).
(B) Localization of Brat mRNA in oocytes by whole-mount in situ
hybridization. Brat mRNA is expressed throughout oogenesis and is
localized to the vegetal pole by stage II. Pigmented oocytes (P) are
shown in the upper panel, animal pole up, to orient the stain relative
to the animal and vegetal pole. Albino oocytes (A) are shown in the
lower panel to clearly display the domain occupied by Brat
transcripts. During the in situ procedure, the cortex was partially
removed from several stage VI oocytes to demonstrate that the bulk
of Brat transcripts are sequestered in the cortex. Arrows indicate the
edge where the cortex was torn off, revealing the underlying,
unstained cytoplasm. (C) Vegetal localization of Brat mRNA in early
cleavage stage embryos. A northern blot of RNA from animal and
vegetal halves of stage 6 embryos. Seven explants or three embryos
were analyzed and the blot was reprobed with Histone H4 to contrast
the localization of Brat mRNA with that of a cytoplasmic mRNA.
Image published in: Horb ME and Thomsen GH (1997)
Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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