XB-IMG-131707
Xenbase Image ID: 131707
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Fig. 4. Analysis of mesoderm
induction by Brat and Xbra in
animal caps. (A) Comparison of
Xbra and Brat activities reveals
that both induce the ventrolateral
markers Xwnt-8 (Christian et al.,
1991) and XMyoD (Frank and
Harland, 1991); only Brat
however, induces the dorsal
mesoderm markers XFKH-1
(Dirkson and Jamrich, 1992) and
chordin (Sasai et al., 1994). (B) A
more detailed examination of
mesoderm induction by Brat. At
low doses of injected mRNA (14-
42 pg), Brat induced ventralposterior
mesoderm, as scored by
the expression of Xwnt-8, Xhox3
(Ruiz i Altaba and Melton, 1989),
Xlhbox6 (Wright et al., 1990), Xbra, and XMyoD. At intermediate doses (42-140 pg) XFKH-1 is induced, followed by organizer-specific genes
goosecoid (gsc) (Cho et al., 1991) and chordin at higher doses (350-700 pg). (C) In tadpole stage 28 animal caps Brat induces globin, a marker
for blood (the most ventral mesoderm derivative), and muscle actin, which marks dorsal mesoderm. Animal caps were injected with synthetic
mRNA at the 2-cell stage and mesoderm induction was analyzed by RT-PCR at either stage 11 (A,B) or stage 28 (C). Image published in: Horb ME and Thomsen GH (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |