Xenbase Image ID: 131708

Fig. 5. Brat induces mesodermal tissues. (A) Control-injected animal caps form solid balls of atypical epidermis. (B) At low doses, 50 pg, Brat-injected animal caps form vesicles, characteristic of ventral mesoderm. Caps in (A) and (B) were scored at stage 28. (C-F) Whole-mount staining for muscle with the 12/101 antibody. (C) Sibling stage 24 whole embryo. Muscle tissue is stained in the segmented somites. (D) CS2-injected animal caps, 250 pg mRNA. No muscle tissue formed, n=11. (E) Brat-injected animal caps, 500 pg mRNA. Muscle formed in 33% of the injected caps, n=27. (F) BVg1-injected animal caps, 5 pg mRNA. Muscle staining is seen in all caps, n=22. Image published in: Horb ME and Thomsen GH (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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