XB-IMG-131710
Xenbase Image ID: 131710
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Fig. 7. Brat is induced by peptide growth factors in the FGF and
TGF-b families. (A) Brat displays dose-dependent induction by FGF
or activin protein. Animal caps were excised at stage 8-9 and
exposed to the indicated concentrations of FGF or activin protein.
Caps were harvested at stage 11, and Brat induction was analyzed on
a northern blot. RNA recovery was equal across the blot (not shown).
(B) Brat is induced by BVg1 and BMP-4. Caps were injected with 30
pg of BVg1 mRNA or 1.8 ng BMP-4 mRNA, excised at stage 9,
harvested at stage 11, and scored for Brat expression by RT-PCR.
EF1-alpha expression is a control for RNA recovery and cDNA
synthesis. Maternal Brat transcripts in the animal pole (see Fig 2c)
account for the background signal in vector-injected animal caps. Image published in: Horb ME and Thomsen GH (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |