Xenbase Image ID: 131710
Fig. 7. Brat is induced by peptide growth factors in the FGF and
TGF-b families. (A) Brat displays dose-dependent induction by FGF
or activin protein. Animal caps were excised at stage 8-9 and
exposed to the indicated concentrations of FGF or activin protein.
Caps were harvested at stage 11, and Brat induction was analyzed on
a northern blot. RNA recovery was equal across the blot (not shown).
(B) Brat is induced by BVg1 and BMP-4. Caps were injected with 30
pg of BVg1 mRNA or 1.8 ng BMP-4 mRNA, excised at stage 9,
harvested at stage 11, and scored for Brat expression by RT-PCR.
EF1-alpha expression is a control for RNA recovery and cDNA
synthesis. Maternal Brat transcripts in the animal pole (see Fig 2c)
account for the background signal in vector-injected animal caps.
Image published in: Horb ME and Thomsen GH (1997)
Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
Printer Friendly View