Xenbase Image ID: 131764

FIG. 3. Activation and suppression of Notch signaling has opposite effects on pronephric duct formation. Opposite effects on GR-Su(H)VP16- (activated) and GR-Su(H)DBM- (dominant negative) injected embryos were observed when examining markers of the pronephric duct by either in situ hybridization or antibody staining using markers of various stages of duct differentiation (c-ret, Lim-1, 4A6 epitope). Injected constructs were induced at stages 19–20 by the addition of dexamethasone. Note that injection of GR-Su(H)VP16 resulted in almost complete obliteration of all three molecular markers of duct morphogenesis on the injected side of the embryos (B, F, J) compared to the uninjected control side (A, E, I). An opposite effect was detected in GR-Su(H)DBM-injected embryos, in which an increase of duct markers was observed on the injected side of the embryos (D, H, L). Black arrow in (J) indicates the location of the missing Lim-1 expression in the pronephric duct. The black arrow in (L) marks the increased expression of Lim-1 in the anterior portion of the injected embryo. Note that the three patches of Lim-1 expression which presumably mark the nephrostomes [yellow arrowheads in (K)] are less distinct after expression of GR-Su(H)DBM [red arrow in (L)]. Image published in: McLaughlin KA et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page