Xenbase Image ID: 131766

FIG. 5. Notch signaling affects expression of markers of the tubules and glomus. Markers of either tubules (Pax-2 or 3G8 epitope) or the glomus (Wt-1) were used to examine the affects of activation or suppression of Notch signaling on kidney organogenesis. (A) The right-hand column shows lateral views of GR-Su(H)VP16- (activated) injected embryos. The left-hand column shows the expression on the uninjected side of embryos. The in situ probe Pax-2 and the 3G8 antibody were used to examine tubule organization and morphogenesis. Pax-2 expression did not show a normal tubule pattern, but instead was expanded in the tubule anlage on the injected side of the embryo. Similarly, the injected side of the embryo displayed disorganized 3G8 monoclonal staining in the tubule anlage compared to the control uninjected side. The developing glomus was examined using the molecular marker Wt-1. The expression of Wt-1 was dramatically increased on the GR-Su(H)VP16-injected side of the embryo and in particular was expanded into a more posterior region. (B) A lateral view of a 3G8 (tubule) antibody stained, GR-Su(H)DBM- (dominant negative) injected embryo. Suppression of the Notch pathway results in normal tubule morphology (blue arrow). It is important to note, however, that extra b-galactosidase-positive cells (magenta-gal pink cells and black arrow) were able to contribute to the region of the developing duct. Image published in: McLaughlin KA et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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