XB-IMG-131815
Xenbase Image ID: 131815
Fig. 3. Expression pattern of X-Delta-2
transcripts in Xenopus embryos. In this and other
figures, embryos and sections are oriented with
anterior on the left. (A-C) Embryos stained in
whole mount for the expression of X-Delta-2
RNA at neural plate stage (A; dorsal view, stage
14), neurulae stage (B; dorsal view, stage 18),
and tailbud stage (C; side view, stage 28). Note
that X-Delta-2 is expressed within the paraxial
mesoderm within stripes of cells that are located
progressively more posterior as development
progresses (arrows). Note also that X-Delta-2
expression occurs in a large domain in the
tailbud. (D) Longitudinal section through
neurulae embryos (stage 18), showing just one
side of the paraxial mesoderm. The three somites
that have formed (labeled a, b and c; demarcated
by arrowheads) do not express X-Delta-2 at
detectable levels. Within the presomitic
mesoderm, however, X-Delta-2 RNA is
expressed in stripes of cells, where the spacing
between each strip (denoted by arrows)
corresponds to about ten cells, and thus the
width of a prospective somite, or somitomere (labeled 1-4; Jacobson and Meier, 1986). Note that X-Delta-2 expression in the youngest
somitomeres (labeled 3 and 4), is broad but then undergoes a refinement, localizing to the anterior edge of the first somitomere (labeled 1).
(E,F) Dorsal view of myogenesis in the paraxial mesoderm of neural tube stage embryos (stage 18) as revealed by the expression of cardiac
actin (E) and of MyoD (F). (G) Expression of X-Notch-1 RNA in the presomitic mesoderm (double arrow) in a cleared, early tadpole embryo
(stage 24), as shown by whole-mount in situ hybridization. (H) Dorsal view of an early neural plate stage embryo (stage 12.5), double-labeled
for the expression of cardiac actin RNA (dark blue) and for X-Delta-2 RNA (light blue). Note that the X-Delta-2 staining (arrow) extends
outside the myogenic domain marked by cardiac actin staining. Image published in: Jen WC et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |