XB-IMG-131817
Xenbase Image ID: 131817
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Fig. 5. Expression of X-Delta-2tr alters the pattern of segmentation in
Xenopus embryos. Embryos injected on one side, at the two-cell
stage, with (A) nlacZ RNA or (B,C) with a mixture of X-Delta-2tr
and nlacZ RNAs. At early tadpole stages, embryos were fixed and
reacted in whole-mount with X-gal, which stains the nuclei blue, and
with the 12/101 antibody using HRP immunohistochemistry, which
stains the muscle cells brown. Shown are representative longitudinal
sections. (A) Embryo injected with just the nlacZ tracer. Note that
each somite consists of mononucleated muscle cells, whose nuclei
line up in a row at the center of each somite. (B) Embryo expressing
X-Delta-2tr RNA with a ‘mild’ phenotype. Note that some of the
myotomal cells have formed somites which are shorter (arrowhead)
or longer than normal (arrow). 12/101 staining on both injected and
uninjected sides is incomplete due to penetration artifacts. In
subsequent experiments using more stringent staining protocols, the
expression of 12/101 was found to be uniform throughout the width
of the somite. (C) Embryo expressing X-Delta-2tr RNA with a
‘strong’ phenotype. Arrow marks the formation of intramyotomal
junction. Image published in: Jen WC et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |