Xenbase Image ID: 131818

Fig. 6. Expression of XSu(H)DBM and X-Delta-2 alters the pattern of segmentation in Xenopus embryos. Embryos were injected on one side with (A) nlacZ RNA, (B) a mixture of nlacZ and XSu(H)DBM RNAs, or (C) a mixture of nlacZ and X-Delta-2 RNAs. Embryos were processed for X-gal and 12/101 staining at early tadpole stages as described in the legend of Fig. 5. In each panel, the left side shows the anterior region of the tissue section where mature somites are located, while the right side shows a posterior view where somites begin to form. (A) Negative control showing the pattern of segmentation is not affected by injection of nlacZ RNA. The nuclei within each somite are normally aligned (arrowhead). (B) Embryo injected with XSu(H)DBM RNA. Note that the onset of 12/101 expression is normal, as shown in the right panel (arrow). However, as somites begin to form, the arrangement of somitic tissue is chaotic, failing to segment properly as shown in the left panel. Intramyotomal junctions appear to form (arrow in left panel) as shown by dense 12/101 staining, but the position and number of these junctions are abnormal. (C) Embryo injected with RNA encoding X-Delta-2. Image published in: Jen WC et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page