Xenbase Image ID: 131819

Fig. 7. The segmental prepattern as marked by the expression of XDelta- 2 is altered by XSu(H)DBM and X-Delta-2tr. Embryos injected with (A) nlacZ RNA, (B) a mixture of nlacZ and XSu(H)DBM RNAs or (C,D) a mixture of nlacZ and X-Delta-2tr RNAs. Embryos were fixed at early neurulae stages, stained for both nlacZ expression with X-gal (light blue), and for X-Delta-2 expression using whole-mount, in situ hybridization (dark blue-purple). (A) Dorsal view of an embryos injected with just nlacZ RNA, with the injected side oriented to the top of the panel. Note the expression pattern of X-Delta-2 demarcated by a double arrow is the same on both sides. (B) Dorsal view of an embryo injected with both nlacZ and XSu(H)DBM RNAs, with the injected side up. Note that on the injected side the expression of XDelta- 2 (demarcated with a double arrow) is not refined into a segmental prepattern, but is uniform within the somitomeric region. (C,D) Embryo injected with a mixture of nlacZ and X-Delta-2tr RNAs. C shows a lateral view of the uninjected side, while D shows a lateral view of the injected side of the same embryo. Double arrow indicates the expression domain of X-Delta-2. Image published in: Jen WC et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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