XB-IMG-131821
Xenbase Image ID: 131821
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Fig. 1. (A) Deletion analysis of ICD. Diagram of the X-Notch-1
intracellular domain (ICD) and of the deletions used for mapping
ESR-1 induction. NLS-Ank contains the SV40 NLS at the Nterminus
(Rupp et al., 1994). The amino acid residues (Coffman et
al., 1990) encompassed by each truncation are indicated. The right
column summarizes the data from RNase protection assays, such as
those shown in Fig. 3, that measure the ability of each construct to
induce ESR-1 expression in neuralized animal caps (+++, 100%; ++,
20-40%; +, 10-20%; +/-, 1-5%; or -, not detectable). RAM23
(Tamura et al., 1995) is the subtransmembrane region. Addition of
the SV40 NLS (Rupp et al., 1994) to the N terminus of ICD22, 23,
24, and 25 did not alter ESR-1 induction. (B) Different forms of XSu(
H)1. Schematic representation of wild-type X-Su(H)1, a form in
which the ankyrin repeats from Notch are fused to the carboxy
terminus (X-Su(H)1/Ank), a form in which four residues required for
DNA binding, between amino acids 193-203, are altered (XSu(
H)1DBM), a truncated form in which the carboxy terminal 117
amino acids are deleted X-Su(H)1-Tr, and a form of X-Su(H)1/Ank
fused to the hormone binding domain of the human glucocorticoid
receptor (hGR). Image published in: Wettstein DA et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |