XB-IMG-131822
Xenbase Image ID: 131822
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Fig. 2. Regions of the X-Notch-1 intracellular domain required for
X-Su(H)1 binding. Lane labels indicate the GST fusion protein (see
Fig. 1A) used to precipitate in vitro-translated [35S]Met-labeled XSu(
H)1. The asterisk marks full-length in vitro-translated X-Su(H)1.
The lane labeled ‘Pre-clear’ (see Methods) shows that GST binds no
X-Su(H)1. The ‘Input’ lane contains 25% of the [35S]Met-labeled XSu(
H)1 used in each binding reaction. Approximate migration of
protein markers is indicated. Longer exposure of the gel shows weak
binding of X-Su(H)1 to GST-Ank, GST-ICD11, and GST-ICD14,
while GST alone still shows no binding. Image published in: Wettstein DA et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |