XB-IMG-131823
Xenbase Image ID: 131823
Fig. 3. Induction of ESR-1 in neuralized animal
caps by ICD and by different forms of XSu(
H)1. Test RNAs (1.0 ng/embryo) along with
Noggin RNA (0.3 ng/embryo) were injected
into two-cell stage embryos. Ectoderm was
excised from blastula embryos (stage 10),
cultured for approximately 6 hours (equivalent
to the neural plate stage), and then assayed by
RNase protection assay for expression of ESR-1
RNA and EF-1a RNA (loading control). Each
assay includes the probes without RNase
treatment (Probes), animals caps injected with
just Noggin RNA (Noggin) and stage-matched
embryo RNA (Embryos). Position of probe
fragments are marked with an arrowhead, while
those of protected fragments are marked with
arrows. (A) ESR-1 RNA levels in neuralized
animal caps expressing different regions of ICD
as shown in Fig. 1A. Note that those lacking
any portion of the ankyrin repeats fail to or
barely induce detectable ESR-1 expression
(lanes 5, 11, 14, 15, and 16). Removing the
RAM23 region (ICD11, lane 13) markedly
reduces the induction of ESR-1 expression
relative to ICD (lane 10). Deletion of sequences downstream of the ankyrin repeats (compare lane 4 to 3) also reduces the induction of ESR-1
expression. (B) ESR-1 RNA levels in neuralized animal caps expressing X-Su(H)1 and X-Su(H)1/Ank (see Fig. 1B). Note that X-Su(H)1 has
undetectable ESR-1-inducing activity (lane 5) and the ankyrin repeats from ICD (NLS-Ank) have very little inducing activity (lane 4), while XSu(
H)1/Ank (lane 7) induces ESR-1 expression to levels found in embryos (lane 8). Image published in: Wettstein DA et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |