Xenbase Image ID: 131826

Fig. 6. The effects of X-Su(H)1, X-Su(H)1/Ank and X-Su(H)1DBM on primary neurogenesis. Albino Xenopus embryos were injected once at the two-cell stage with test RNAs (1.0 ng/embryo) along with lacZ RNA (0.2 ng/embryo). At neural plate stages, the embryos were processed both for b-galactosidase expression (light blue) to mark the injected side, and for the expression of N-tubulin (dark purple) to mark the formation of primary neurons. Primary neurons arise in three stripes on either side of the midline of the neural plate: a long medial stripe, a short more anterior intermediate stripe, and a wide lateral stripe. Shown are dorsal views, with anterior to the left and the injected side oriented up in each panel. Embryos shown in each row are from the same experiment. Expression of: (A) wild-type X-Su(H)1 has little or no effect on the formation of primary neurons; (B) X-Su(H)1/Ank abolishes primary neurons; (C,F) X-Su(H)1DBM produces a neurogenic phenotype; (D) X-Su(H)1 along with X-Su(H)1DBM ameliorates the neurogenic phenotype; (E) X-Su(H)1/Ank along with X-Su(H)1DBM completely reverses the neurogenic phenotype; (G)X-Delta-1 reduces the number of primary neurons; (H) X-Su(H)1 along with X-Delta-1 partially reverses the effects of X-Delta-1; and (I) X-Su(H)1DBM along with X-Delta-1 not only reverses the effects of X-Delta-1 but also results in a neurogenic phenotype. Image published in: Wettstein DA et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue rbpj.S CBF-1, CBF1, csl, lag-1, rbpsuh, Su(H), suppressor of hairless, X-Su(H), XSu(H) X. laevis Sometime during NF stage 15 to NF stage 16 neural plate Rohon-Beard neuron neuron Image source: Published Larger Image Printer Friendly View

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