Xenbase Image ID: 131833

Fig. 6. Fate mapping of midline cells in the retina field of Xenopus embryos. A to D show anterior views. (A) A diagram showing that midline cells in the retina field were labeled by DiI at stages 12 or 12.5 and followed to stage 25 to determine whether the midline cells migrate to the lateral regions. (B) The original location of DiI-labeled cells. This embryo was labeled with DiI at stage 12 and fixed at stage 12.5. DiI was photoconverted to produce the brown spot in the middle of the retina field, which was revealed by in situ hybridization with the Xenopus Pax-6 probe. (C) An image of a stage 25 embryo illuminated by incident light. A yellow arrow points to an eye. (D) A composite of the image shown in C and an image of the same embryo viewed under rhodamine optics to map the location of the fluorescent cells. A yellow arrow points to an eye. This embryo was previously labeled with DiI at stage 12.5. Results were similar with embryos labeled at stage 12. (E) A left side view of another DiI-labeled embryo. Note the absence of DiI labeled cells in the left retina. A white arrow points to DiI-labeled cells. A yellow arrow points to an eye. Image published in: Li H et al. (1997) Copyright © 1997. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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