Xenbase Image ID: 131865

Figure 4. Estimation of Protein Abundance in the Xenopus Egg (A) Previously published protein concentrations for 49 proteins versus measured ion current in MS1 spectrum normalized to protein length. The Pearson correlation is 0.92. On average, the predicted protein concentration is approximately 2-fold different from the reported protein concentration. (B) Histogram of concentration for all identified proteins regressed from normalizedMS1 ion current.Median concentration of measured proteins is approximately 30 nM. (C) Estimated concentration for subunits of stable complexes is similar. For the APC/C, we additionally distinguished between subunits that were reported to be dimeric (square) or monomeric (triangle) within the complex. Although our accuracy is not good enough to separate the two populations, the estimated concentrations for dimeric subunits tend to be higher than those for monomeric subunits. (D) Concentrations for enzymes of a metabolic pathway can vary widely. For each metabolic pathway, the predicted concentrations of its members are plotted (based on the Kyoto Encyclopedia of Genes and Genomes). Image published in: Wühr M et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page