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XB-IMG-131867

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Fig. 1. VegT contains a T-domain found in proteins from C. elegans, Drosophila, Xenopus and mammals and is expressed both maternally and zygotically. (A) Alignment of VegT with the T-box DNAbinding domain in other proteins. mTbx2, mouse Tbox2; omb, Drosophila optomotor blind; F21H11.3, presumptive protein from the Caenorhabditis genome project; VegT, Xenopus VegT; Xbra, Xenopus Brachyury. Identical residues are shown on a black background. (B) Vegetally localized expression of VegT. The total RNA equivalent of five stage VI oocyte halves was loaded on each lane and hybridized with VegT and FGFR probes. V, vegetal; A, animal; T, whole embryo. (C) Developmental expression of VegT. The RNA equivalent of 3 embryos was loaded per lane. OV, ovulated egg; 4, 4-cell embryos; other staging according to Nieuwkoop and Faber (1976). In B and C, FGFR expression was used as a control for RNA loading.

Image published in: Zhang J and King ML (1996)

Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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