Xenbase Image ID: 131869

Fig. 3. VegT can function as a transcription factor. (A) Nuclear localization of myc-tagged VegT protein. Single blastomere of a 2- cell embryo was injected at the animal pole with MT-VegT RNA. Shown is a stage 9 embryo stained with myc antibody 9E10. Progeny of the uninjected blastomere are on the right and served as a negative control. (B) Yeast transcription reporter assay. Three colonies from each transformation group were transferred to a filter and stained with X-gal. Each construct contains the Gal4 DNA-binding domain fused with either vector alone (pGBT), VegT C-terminal 176 amino acids (pGBT/VT-AD), or the Gal4 activation domain (pGBT/G-AD). Image published in: Zhang J and King ML (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page