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Fig. 6. Ectopic effect of VegT injection on animal cap explants and whole embryos. VegT RNA was injected into the animal pole of both cells at the 2- cell stage. (A-E) Animal caps were explanted at blastula-stage (stage 8) and cultured to the equivalent of stage 41. (A,B) show cap morphology. (C-E) are histological sections. (A) Control animal caps form ciliated epidermis. (B) VegT-injected animal caps form swelled vesicles, a phenotype of ventral mesoderm. (C) Control animal caps showing atypical epidermis. (D) VegT RNA-injected cap showing block of muscle cells. (E) Another example of VegT RNA-injected cap where extensive ventral mesoderm including mesenchyme and blood were formed. (F-H) Alternatively, RNAs were injected into the animal pole at the 2-cell stage and the embryos were cultured to stage 11. (F) Stage 11.5 embryo. VegT-DAD-injected control together with VegT-injected sibling. Note the failure of the blastopore to close. (G) Section of stage 11.5 embryo injected with 0.7 ng VegT RNA showing collapse of the blastocoel. (H) Section of stage 11.5 embryo injected with 2.0 ng VegT RNA showing the complete loss of the blastocoel. Note the protrusive activity of the ectoderm in G and H. Image published in: Zhang J and King ML (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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