XB-IMG-131873
Xenbase Image ID: 131873
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Fig. 7. VegT can activate expression of Xwnt-8 and itself in animal
caps. 2-cell-stage embryos were injected with 2 ng VegTD 3'UTR
RNAs at the animal pole. At stage 8, the animal caps were explanted
and cultured until control embryos reached stage 11. Total RNA was
isolated from twenty animal caps (An caps) and assayed by northern
blot hybridization with VegT (left panel) or Xwnt-8 (right panel) as
probes. VegT, endogenous VegT RNAs; VegTD3¢UTR, injected
VegT RNA; un-inj., uninjected; inj., injected; WE, whole embryos.
EF1a was used as an RNA loading control. Image published in: Zhang J and King ML (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |