XB-IMG-131874
Xenbase Image ID: 131874
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Fig. 8. Ectopic expression of VegT in dorsal/animal blastomeres
suppresses head formation. At the 8-cell stage, 1 ng of RNA was injected
into each dorsal/animal blastomere. The embryos were allowed to
develop to stage 34 for morphological assay (A,B) or to stage 28 for
whole-mount in situ hybridization with digoxigenin-labeled En-2 RNA
probe (C,D). (A) Control embryos injected with VegTDAD or buffer.
(B) Embryos injected with VegT. (C) Control embryos showing dark-blue
staining of En-2 transcripts (arrowheads). Darkly pigmented cement
glands are also indicated (open arrows). (D) VegT-injected embryos
showing variation of En-2 expression. The embryo on the top shows no
En-2 expression, while the bottom one has normal expression of En-2,
although it lacks cement gland and eyes. Image published in: Zhang J and King ML (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |