XB-IMG-131930
Xenbase Image ID: 131930
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Fig. 3. (A) Animal caps from b-catenin overexpressing embryos produce a ‘dorsal signal’ but will not induce animal caps to form mesoderm.
The design of the experiments are described in the text and illustrated here diagrammatically. WT, wild type; b cat++, overexpressing b-catenin
mRNA; b cat-, b-catenin-depleted by the injection of antisense oligo into oocytes. RNA was prepared from groups of 5 marginal zones, 10
animal caps or 10 animal/vegetal recombinants at the late neurula stage, and northern blots were probed for the dorsal mesodermal marker
MyoD. Ef1a was used as a loading control. (B) Overexpression of b-catenin in animal caps causes them to be dorsalized. Nieuwkoop
recombinants consisiting of either wild-type caps and wild-type vegetal masses (Bi), or b-catenin-overexpressing caps and wild-type vegetal
masses (Bii) were made at the midblastula stage and cultured until siblings reached the neurula stage (stage 16). Overexpressing caps elongated
dramatically compared to controls. Image published in: Wylie C et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |