Xenbase Image ID: 131934

Fig. 7. Only GSK is unable to cause the expression of dorsal mesodermal and neural markers in b-catenin-deficient embryos. This is a northern blot of RNA from neurulae derived from embryos untreated and treated with ologo. The blot was probed for the dorsal markers MyoD (skeletal muscle) and NRP1(neural tissue), the ventral marker Xwnt8 and the loading control Ef1a. Each lane is described both in terms of the treatment each sample had as oocytes (i.e. injected with oligo or not) and in terms of the treatment after fertilization (ie. the injection of a specific mRNA at the 8-16 cell stage or not). This information is also summarized above each figure as oocyte treatment: U, uninjected or O, antisense oligo-injected; and embryo treatment: b, b-catenin mRNA-injected; V, BVg1; G, dn-gsk; B, tBR; S, siamois; N, noggin. Lanes 21-24 are of animal cap-derived tissue rather than whole embryos. F, bFGF-treated caps. Image published in: Wylie C et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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