XB-IMG-131965
Xenbase Image ID: 131965
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Fig. 10. In situ hybridization analysis of embryos injected with RNA
encoding Xbra-EnR. (A–D) Embryos hybridised with an Xbra probe
at stage 10 (A,B) and stage 14 (C,D). Control embryos at the early
gastrula stage express Xbra throughout the marginal zone (A).
Control embryos at the neurula stage express Xbra in the notochord
and in posterior cells (C). Embryos injected with RNA encoding
Xbra-EnR do not express Xbra in the organizer at the early gastrula
stage (B; see left-hand side of embryo) or in the notochord at the
neurula stage (D). The greater intensity of staining in the ventral
region of the embryo shown in B is not a consistent observation and
decreases in endogenous levels of Xbra have been confirmed by
RNAase protection (not shown). (E–H) Embryos hybridised with a
Pintallavis probe at stage 10 (E,F) and stage 14 (G,H). Expression of
Pintallavis in embryos injected with RNA encoding Xbra-EnR (F,H)
is similar to that in controls (E,G). Image published in: Conlon FL et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |