XB-IMG-131968
Xenbase Image ID: 131968
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FIG. 3. The temporal expression of early mesodermal genes, neural genes, and genes that mediate neural induction are unaffected in
antisense a6 integrin-treated embryos. Control embryos and embryos injected with antisense a6 integrin constructs that displayed
neurulation arrest were evaluated semiquantitatively by RT-PCR. Equivalent RNA samples isolated from neural plate (12 and 13),
neurula (15, 18, 20, and 25), and tail bud (30, 32, and 35), as well as antisense a6 integrin-treated embryos (stages 13, 20, and 32) were
used as templates for cDNAs. Each sample was subjected to RT-PCR using primers specific for (A) a6 integrin, (B) the mesodermally
expressed gene Xbra, and (C) the neurally expressed genes N-CAM and neuroD and genes involved in neural induction: BMP4, noggin,
follistatin, and chordin. Parallel RT-PCRs were performed using primers specific for b1 integrin to normalize for RNA content. Image published in: Lallier TE and DeSimone DW (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |