Xenbase Image ID: 132014

Fig. 5. Expression of Xom is prevented by dispersion of Xenopus embryos. Addition of activin or BMP-4, but not FGF, restores expression. Xenopus embryos were transferred to calcium- and magnesium-free medium at stage 7.5, their vitelline membranes were removed, and the blastomeres were kept dispersed by passing a gentle stream of medium over the cells from a Pasteur pipette. Dispersed blastomeres were cultured to stage 10.5 in the presence of the indicated factors and analysed for expression of Xom by RNAase protection. EF-1a was used as a loading control. Lane 1, intact control embryos; lane 2, dispersed blastomeres with no additional factors; lane 3, dispersed blastomeres plus 50 ng/ml FGF-2; lane 4, dispersed blastomeres plus 100 ng/ml BMP-4; lane 5, dispersed blastomeres plus 8 units/ml activin. Image published in: Ladher R et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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