Xenbase Image ID: 132015

Fig. 6. Activation of Xom expression by activin and BMP-4 is not inhibited by cycloheximide. Xenopus embryos were transferred to calcium- and magnesium-free medium at stage 7.5, their vitelline membranes were removed, and the blastomeres were kept dispersed by passing a gentle stream of medium over the cells from a Pasteur pipette. Dispersed blastomeres were cultured to stage 10.5 in the absence of additional factors (lanes 1 and 2) or in the presence of FGF-2 (lanes 3 and 4), BMP-4 (lanes 5 and 6) or activin (lanes 7 and 8). Samples in even-numbered lanes were cultured in the continual presence of 7.5 mg ml-1 cycloheximide. This was sufficient to reduce incorporation of [35S]methionine into trichloroacetic acid-insoluble material by over 94%. Expression of Xom was analysed by RNAase protection. EF-1a was used as a loading control. Image published in: Ladher R et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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