XB-IMG-132019
Xenbase Image ID: 132019
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Fig. 10. Injection of RNA encoding Xom into dorsal blastomeres of
Xenopus embryos causes loss of anterior structures and of the
notochord. Xom RNA (4 ng total) was injected into the two dorsal
blastomeres of Xenopus embryos at the 4-cell stage, and the embryos
were allowed to develop to stage 32. Control embryos received
injections of RNA encoding DXom, which encodes a truncated
version of Xom. (A) Embryos expressing DXom develop normally.
(B) Embryos injected with RNA encoding Xom lack anterior
structures. (C) Whole-mount antibody staining using the monoclonal
antibody MZ15 demonstrates that embryos injected with DXom RNA
form a notochord. (D) Embryos injected with RNA encoding Xom
lack a notochord. Image published in: Ladher R et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |