Xenbase Image ID: 132096

Fig. 2. Effects of XASH-3 and NeuroD on the expression of X-Delta-1, X-Notch-1 and N-tubulin in neuralized animal caps. (A) Animal caps were isolated at blastula stages from embryos injected at the twocell stage with different RNAs, cultured to the equivalent of stage 14 (neural plate) and then assayed for the expression of XDelta- 1 and X-Notch-1 using RNAse protection. EF-1a is a ubiquitously expressed RNA and serves as a loading control (Krieg et al., 1989). Note that, in animal caps neuralized by Noggin (Lamb et al., 1993), the expression of X-Delta-1 is activated by both NeuroD and XASH-3, and this can be suppressed by the inclusion of the activated form of X-Notch-1 called ICD (Chitnis et al., 1995). (B) Animal caps were isolated at blastula stages from embryos injected at the two-cell stage with different RNAs, cultured to the equivalent of stage 16 (late neural plate) and then assayed for the expression of NCAM (Kintner and Melton, 1987) or for N-tubulin (Richter et al., 1988) using RNAse protection assays. Note that NeuroD induces the expression of N-tubulin in animal caps neuralized by Noggin, as shown by the expression of NCAM, and that this activation is not blocked by the coinjection of ICD. Image published in: Chitnis A and Kintner C (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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