Xenbase Image ID: 132148

Fig. 1. Autonomous expression of endodermal markers. (A) Animal caps or vegetal poles were dissected from blastula embryos (stage 8-9) as shown. Vegetal pole explants were also dissected into dorsal and ventral halves. Explants were cultured in isolation until midtailbud stages (stages 33-36). RNA levels were assayed by RT-PCR. (B) Vegetal pole explants express Xlhbox-8, IFABP and insulin, but not the mesodermal markers cardiac actin, Xtwist or globin. Animal caps do not autonomously express either mesodermal or endodermal markers. (C) The capacity for Xlhbox-8 expression is localized to the dorsal side of the vegetal pole whereas IFABP expression is seen in both the dorsal and ventral vegetal pole explants. WE lanes represent RT-PCR products where mRNA from an unmanipulated embryo was used for cDNA synthesis. The WE-RT lanes represent assays in which reverse transcriptase was left out of the cDNA synthesis step with whole embryo RNA as a template. This serves as a control for DNA contamination. Elongation factor 1 alpha (EF-1a) is included as a control to insure that similar amounts of RNA are used in each lane. Image published in: Henry GL et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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