Xenbase Image ID: 132152

Fig. 5. (A) Explants dissected from UV-ventralized blastula embryos fail to express Xlhbox-8 but express normal levels of IFABP. The dorsoanterior index (D.A.I.) was determined at middle tailbud stages and in the experiment shown the UV irradiation was sufficient to give a D.A.I. of zero (explants dissected from embryos with a D.A.I. of 2, 3 and 4 all express Xlhbox-8). Explants dissected from embryos that were UV irradiated during the first cell cycle, followed by injection of 50 picograms of B-Vg1 mRNA into a single vegetal blastomere at the 8-cell stage express both Xlhbox-8 and IFABP. In UV-ventralized whole embryos both Xlhbox-8 and IFABP are not expressed. Injection of B-Vg1 recovers IFABP and Xlhbox-8 expression in whole UV embryos. (B) Endodermal explants dissected from embryos injected at the 1-cell stage, into the vegetal pole, with 80 picograms of B-Vg1 mRNA. A slight increase in Xlhbox-8 expression results with no effect on IFABP expression. As a control, in the same experiment animal caps were dissected from embryos that were injected into the animal pole with 80 picograms of B-Vg1 mRNA at the one cell stage. WE lanes represent RT-PCR products where mRNA from an unmanipulated embryo was used for cDNA synthesis. The WE-RT lanes represent assays in which reverse transcriptase was left out of the cDNA synthesis step with whole embryo RNA as a template. This serves as a control for DNA contamination. Elongation factor 1 alpha (EF-1a) is included as a control to insure that similar amounts of RNA are used in each lane. Image published in: Henry GL et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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