XB-IMG-132161
Xenbase Image ID: 132161
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Fig. 1. XlHbox 8 encodes a novel type of
homeodomain protein. (A) Construction of
XlHbox 8 fusion protein. The top line shows the
genomic EcoRl fragment (E-E) that carries the
3'-most two-thirds of the homeobox and
downstream sequences. The far left end shows
the rest of the homeobox to indicate the position
of the EcoRl site within the homeobox. The
middle line shows the Alul/Sau3A\ fragment
(A-S) used in constructing the /3-gal fusion
protein in pTRB 2 (bottom line). The proteincoding
region is indicated by an open box, the
homeobox by a shaded portion within this. The
translation termination codon is asterisked. (B)
Nucleotide and amino acid sequence of XlHbox
8. The sequence between the EcoRl and the
Haelll site was determined on both strands (see
Methods). Some additional downstream sequence
is shown to indicate the Sau3AI site used in
constructing the fusion protein. A Pstl site used
in determining orientation in pTRB 2 is also
shown. The homeodomain is indicated in bold
type and a sequence of eight amino acids also
found in the 'potentiator' region of human
glucocorticoid receptor is underlined. (C)
Comparison of XlHbox 8 with other
homeodomain sequences. The relevant region of
the prototype Antp homeodomain (amino acid
position within the 60 residues is indicated above
the sequences) is compared first with XlHbox 2
(Wright et al. 1987) and then with XlHbox 8. The
position of the helix-turn-DNA-recognition
helix motif is indicated (De Robertis et al. 1985;
Scott et al. L988). The corresponding part of five
other major classes of homeodomain are also
presented. Image published in: Wright CV et al. (1989) Copyright © 1989. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |