XB-IMG-132162
Xenbase Image ID: 132162
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Fig. 2. Spatial distribution of XlHbox 8 transcripts.
(A) Stage 38 Xenopus embryos were dissected into five
parts (1-5) and RNA extracted from each set of pooled
fragments for analysis by RNAse protection as shown below
(for full description see Methods). (B) RNAse protection of
XlHbox 8 probe by total RNA from whole tadpoles or
fragments thereof. Lane assignments are: P, probe
incubated alone without RNAse; —, no RNA control; K,
10 j.ig adult kidney RNA control; 1-5, total RNA from 15
fragments of each type indicated in panel A; W, total RNA
from 10 whole tadpoles. Probe length is indicated; fulllength
protection is at 139 nucleotides (nt). The other major
bands (about 8-10 nt shorter) probably represent artefactual
cutting within the RNA duplex (see Wright et al. 1987), but
could be due to transcripts from other copies of the XlHbox
8 gene within the tetraploid X. laevis genome with minor
allelic variations (see Fritz et al. 1988). The film was
deliberately over-exposed as a stringent test of the
restriction of XlHbox 8 RNA to fragment 4. (C) RNAse
protection of cytoskeletal actin probe by total RNA from
the same samples. Lane assignments are exactly as for panel
B, except that total RNA from only 9 fragments (lanes 1-5)
or 6 whole tadpoles (lane W) was analysed. Probe length is
indicated and the position of the cognate cytoskeletal actin
band is shown. The film was exposed for approx. 2h to
allow the band in fragment 5 to show properly - for this
reason the intensities of the bands in lanes K, 1 and W are
well outside the linear range of the film. From other
exposures, the band intensities are in good agreement with
the recovery of total RNA as judged by agarose gel
electrophoresis. Markers are single-strand sequence ladders
from an M13 subclone of XlHbox 2 (Wright et al. 1987). Image published in: Wright CV et al. (1989) Copyright © 1989. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |