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FIG. 4. Induction of Xbra and Xbra3 in isolated animal caps by mesoderm-inducing factors. Caps were isolated at stage 9 and treated with either activin or FGF at concentrations from 50 to 3 z 125 ng/ml. RNA was extracted and analysed by RT-PCR at the stages shown. All assays were controlled for linearity of the amplification. Analysis at stage 12 shows that both Xbra and Xbra3 are induced by activin and FGF, with Xbra showing a lower threshold for induction by FGF. At stage 25, a clear difference is seen between Xbra and Xbra3 in the response to both growth factors. Expression of Xbra3 is clearly maintained at high levels, whereas Xbra transcripts are barely detectable. Image published in: Strong CF et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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